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Addgene

hTspan12(1-305)-1D4_pTT5
(Plasmid #216381)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216381 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTT5
  • Backbone manufacturer
    NRC Biotechnology Research Institute (Yves Durocher)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tetraspanin-12
  • Alt name
    Tspan12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Entrez Gene
    TSPAN12 (a.k.a. EVR5, NET-2, NET2, TM4SF12)
  • Promoter CMV
  • Tag / Fusion Protein
    • Rho1D4 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNASU (HsCD00045716)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.03.578714 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hTspan12(1-305)-1D4_pTT5 was a gift from William Weis (Addgene plasmid # 216381 ; http://n2t.net/addgene:216381 ; RRID:Addgene_216381)

  • For your References section:

    The co-receptor Tspan12 directly captures Norrin to promote ligand-specific β-catenin signaling. Bruguera ES, Mahoney JP, Weis WI. bioRxiv 2024.02.03.578714 10.1101/2024.02.03.578714
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