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FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
(Plasmid #216378)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216378 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Gibco
  • Vector type
    Insect Expression ; Baculovirus
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Frizzled-4
  • Alt name
    Fzd4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1600
  • Entrez Gene
    Fzd4 (a.k.a. Fz4)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • HA signal sequence-FLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1 was a gift from William Weis (Addgene plasmid # 216378 ; http://n2t.net/addgene:216378 ; RRID:Addgene_216378)

  • For your References section:

    PI(4,5)P(2)-stimulated positive feedback drives the recruitment of Dishevelled to Frizzled in Wnt-beta-catenin signaling. Mahoney JP, Bruguera ES, Vasishtha M, Killingsworth LB, Kyaw S, Weis WI. Sci Signal. 2022 Aug 23;15(748):eabo2820. doi: 10.1126/scisignal.abo2820. Epub 2022 Aug 23. 10.1126/scisignal.abo2820 PubMed 35998232
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