Skip to main content
Addgene

FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual
(Plasmid #216377)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216377 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFastBacDual
  • Backbone manufacturer
    Gibco
  • Vector type
    Insect Expression ; Baculovirus
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LDL receptor related protein 6
  • Alt name
    LRP6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5000
  • Entrez Gene
    LRP6 (a.k.a. ADCAD2, STHAG7)
  • Promoter Polyhedrin (LRP6)/p10 (MESD)
  • Tags / Fusion Proteins
    • HA signal sequence-FLAG-3C cleavage site (N terminal on insert)
    • 9xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual was a gift from William Weis (Addgene plasmid # 216377 ; http://n2t.net/addgene:216377 ; RRID:Addgene_216377)
Related items:
Commonly requested with: