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Addgene

pCMV-tomm20-ECFP-RspANFAST
(Plasmid #216348)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216348 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone manufacturer
    Twist
  • Backbone size w/o insert (bp) 4181
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tomm20-ECFP-RspANFAST
  • Species
    RspAFAST derived from photoactive yellow protein from Rheinheimera sp A13L and ECFP from GFP from A. victoria
  • Insert Size (bp)
    1206
  • Promoter CMV
  • Tag / Fusion Protein
    • tomm20 (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-tomm20-ECFP-RspANFAST was a gift from Alison Tebo (Addgene plasmid # 216348 ; http://n2t.net/addgene:216348 ; RRID:Addgene_216348)
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