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Addgene

ER-FAST-IRES-iRFP670
(Plasmid #216344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene #70113
  • Backbone size w/o insert (bp) 3989
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRL_pFAST-IRES-CANX_iRFP670
  • Species
    pFAST derived from photoactive yellow protein (PYP) from H. Halophila; iRPF670 derived from Rhodopseudomonas palustris CGA009
  • Insert Size (bp)
    3944
  • Mutation
    n/a
  • Promoter CMV
  • Tag / Fusion Protein
    • myc for FAST and iRF670 proteins (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation 407F to L within Calnexin also works

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ER-FAST-IRES-iRFP670 was a gift from Alison Tebo (Addgene plasmid # 216344 ; http://n2t.net/addgene:216344 ; RRID:Addgene_216344)
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