Peroxisomes-FAST
(Plasmid
#216343)
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PurposeFor testing localization of pFAST protein to peroxisomes
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAddgene #70113
- Backbone size w/o insert (bp) 3846
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepFAST_SKL
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SpeciespFASTderived from photoactive yellow protein (PYP) from H. Halophila
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Insert Size (bp)429
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Mutationn/a
- Promoter CMV
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Tag
/ Fusion Protein
- myc for FAST protein (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Peroxisomes-FAST was a gift from Alison Tebo (Addgene plasmid # 216343 ; http://n2t.net/addgene:216343 ; RRID:Addgene_216343)