pEutRwc
(Plasmid
#216334)
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PurposeAn IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216334 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB3T5
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameEutR
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SpeciesEscherichia coli
Cloning Information for Gene/Insert 1
- Cloning method Gibson Cloning
Gene/Insert 2
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Gene/Insert nameSuperfolder green fluorescent protein
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Alt namesfGFP
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEutRwc was a gift from Mark Styczynski (Addgene plasmid # 216334 ; http://n2t.net/addgene:216334 ; RRID:Addgene_216334) -
For your References section:
Active Analyte Import Improves the Dynamic Range and Sensitivity of a Vitamin B(12) Biosensor. McNerney MP, Piorino F, Michel CL, Styczynski MP. ACS Synth Biol. 2020 Feb 21;9(2):402-411. doi: 10.1021/acssynbio.9b00429. Epub 2020 Feb 13. 10.1021/acssynbio.9b00429 PubMed 31977200