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pCMV5-FLAG-Gene33
(Plasmid #21633)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21633 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    Dr. David Russell, UTSW
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gene33
  • Alt name
    Mig6, Ralt, ERRFI
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1380
  • GenBank ID
    x07266
  • Entrez Gene
    Errfi1 (a.k.a. RGD1307599, Ralt, Xxx, gene 33)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer CMV Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid was also used in Xu et al. 2005

the construct can be used in cardiomyocytes

There is a V to L amino acid change at position 6. The lab feels it is conservative enough not to matter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-FLAG-Gene33 was a gift from John Kyriakis (Addgene plasmid # 21633 ; http://n2t.net/addgene:21633 ; RRID:Addgene_21633)

  • For your References section:

    Gene 33/Mig-6, a transcriptionally inducible adapter protein that binds GTP-Cdc42 and activates SAPK/JNK. A potential marker transcript for chronic pathologic conditions, such as diabetic nephropathy. Possible role in the response to persistent stress. Makkinje A, Quinn DA, Chen A, Cadilla CL, Force T, Bonventre JV, Kyriakis JM. J Biol Chem. 2000 Jun 9. 275(23):17838-47. 10.1074/jbc.M909735199 PubMed 10749885
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