pUlaR
(Plasmid
#216327)
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PurposeT7 promoter driving expression of ulaR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216327 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameL-Ascorbate-6-P-responsive transciptional repressor
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Alt nameUlaR
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SpeciesEscherichia coli
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUlaR was a gift from Mark Styczynski (Addgene plasmid # 216327 ; http://n2t.net/addgene:216327 ; RRID:Addgene_216327) -
For your References section:
Harnessing Escherichia coli's Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency. Piorino F, Styczynski MP. ACS Synth Biol. 2022 Nov 18;11(11):3592-3600. doi: 10.1021/acssynbio.2c00335. Epub 2022 Oct 27. 10.1021/acssynbio.2c00335 PubMed 36300901