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Addgene

pCF
(Plasmid #216326)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216326 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUC18
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Superfolder green fluorescent protein
  • Alt name
    sfGFP
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF was a gift from Mark Styczynski (Addgene plasmid # 216326 ; http://n2t.net/addgene:216326 ; RRID:Addgene_216326)
  • For your References section:

    Harnessing Escherichia coli's Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency. Piorino F, Styczynski MP. ACS Synth Biol. 2022 Nov 18;11(11):3592-3600. doi: 10.1021/acssynbio.2c00335. Epub 2022 Oct 27. 10.1021/acssynbio.2c00335 PubMed 36300901