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Addgene

pCEP4-HA-MEKK1-1-1493
(Plasmid #21632)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21632 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEP4
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 10400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitogen-activated protein kinase kinase kinase 1
  • Alt name
    MEKK1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1500
  • GenBank ID
    NM_053887
  • Entrez Gene
    Map3k1 (a.k.a. Mekk1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nhe I (unknown if destroyed)
  • 3′ cloning site Bam HI (unknown if destroyed)
  • 5′ sequencing primer pCEP forward
  • 3′ sequencing primer EBV Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

full length MEKK1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEP4-HA-MEKK1-1-1493 was a gift from Melanie Cobb (Addgene plasmid # 21632 ; http://n2t.net/addgene:21632 ; RRID:Addgene_21632)

  • For your References section:

    MEKK1 phosphorylates MEK1 and MEK2 but does not cause activation of mitogen-activated protein kinase. Xu S, Robbins D, Frost J, Dang A, Lange-Carter C, Cobb MH. Proc Natl Acad Sci U S A. 1995 Jul 18. 92(15):6808-12. 10.1073/pnas.92.15.6808 PubMed 7624324
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