pET28a-nanoHER2-SpyTag003
(Plasmid
#216312)
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PurposeExpresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216312 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 5809
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenanoHER2-SpyTag003
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SpeciesSynthetic
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Insert Size (bp)444
- Promoter T7 promoter
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Tags
/ Fusion Proteins
- SpyTag003 (C terminal on insert)
- His6 tag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-nanoHER2-SpyTag003 was a gift from Mark Howarth (Addgene plasmid # 216312 ; http://n2t.net/addgene:216312 ; RRID:Addgene_216312) -
For your References section:
SpyMask enables combinatorial assembly of bispecific binders. Driscoll CL, Keeble AH, Howarth MR. Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9. 10.1038/s41467-024-46599-9 PubMed 38493197
