pET28-SpyCatcher002-MBP
(Plasmid
#216295)
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PurposeExpresses SpyCatcher002 linked to maltose-binding protein in bacterial cells
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 6917
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpyCatcher002-MBP
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SpeciesSynthetic
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Insert Size (bp)1548
- Promoter T7 promoter
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Tags
/ Fusion Proteins
- His6 tag (N terminal on insert)
- TEV cleavage site (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-SpyCatcher002-MBP was a gift from Mark Howarth (Addgene plasmid # 216295 ; http://n2t.net/addgene:216295 ; RRID:Addgene_216295) -
For your References section:
SpyMask enables combinatorial assembly of bispecific binders. Driscoll CL, Keeble AH, Howarth MR. Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9. 10.1038/s41467-024-46599-9 PubMed 38493197
