pET21b-MBPx-MBPx-SpyTag003
(Plasmid
#216291)
-
PurposeExpresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216291 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET21b
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 7726
-
Vector typeBacterial Expression, Affinity Reagent/ Antibody
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMBPx-MBPx-SpyTag003
-
Alt name(MBPx)2-SpyTag003
-
SpeciesSynthetic
-
Insert Size (bp)381
-
GenBank ID
- Promoter T7 promoter
-
Tags
/ Fusion Proteins
- His6 (N terminal on insert)
- SpyTag003 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET21b-MBPx-MBPx-SpyTag003 was a gift from Mark Howarth (Addgene plasmid # 216291 ; http://n2t.net/addgene:216291 ; RRID:Addgene_216291) -
For your References section:
SpyMask enables combinatorial assembly of bispecific binders. Driscoll CL, Keeble AH, Howarth MR. Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9. 10.1038/s41467-024-46599-9 PubMed 38493197
