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Addgene

pMIG-mGATA3
(Plasmid #21629)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21629 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMIG-W
  • Backbone manufacturer
    Plasmid #12282
  • Backbone size w/o insert (bp) 7166
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Slow growing--may need to incubate for up to 48hrs
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GATA3
  • Alt name
    GATA binding protein 3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1300
  • Mutation
    None
  • GenBank ID
    NM_008091
  • Entrez Gene
    Gata3 (a.k.a. Gata-3, jal)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pLXSN 5'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Zhang, D.H., Yang, L., Cohn, L., Parkyn, L., Homer, R., Ray, P., and Ray, A. (1999). Inhibition of allergic inflammation in a murine model of asthma by expression of a dominant-negative mutant of GATA-3. Immunity 11, 473–482.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIG-mGATA3 was a gift from Zena Werb (Addgene plasmid # 21629)

  • For your References section:

    GATA-3 links tumor differentiation and dissemination in a luminal breast cancer model. Kouros-Mehr H, Bechis SK, Slorach EM, Littlepage LE, Egeblad M, Ewald AJ, Pai SY, Ho IC, Werb Z. Cancer Cell. 2008 Feb . 13(2):141-52. 10.1016/j.ccr.2008.01.011 PubMed 18242514
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