pDEST14-DoubleCatcher-β-Lock
(Plasmid #216287)

• Purpose: Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.
• Depositing Lab: Mark Howarth
• Publication: Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9.

Backbone

• Vector backbone: pDEST14
• Backbone manufacturer: Thermo Fisher Scientific
• Backbone size w/o insert (bp): 6422
• Total vector size (bp): 7301
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DoubleCatcher β-Lock
• Species: Synthetic
• Insert Size (bp): 879
• Promoter: T7 promoter
• Tags / Fusion Proteins:
  • His6 (N terminal on insert)
  • TEV cleavage site (N terminal on insert)
  • TEV cleavage site (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry