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pDEST14-SpyCatcher003-TEVs-SpyTag003DA
(Plasmid #216283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST14
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 6422
  • Total vector size (bp) 6909
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCatcher003-TEVs-SpyTag003DA
  • Alt name
    Masked SpyCatcher003
  • Alt name
    SpyCatcher003-TEV site-SpyTag003DA
  • Alt name
    SpyCatcher003-TEVs-SpyTag003D117A
  • Species
    Synthetic
  • Insert Size (bp)
    489
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • His6 (N terminal on insert)
    • TEV cleavage site (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST14-SpyCatcher003-TEVs-SpyTag003DA was a gift from Mark Howarth (Addgene plasmid # 216283 ; http://n2t.net/addgene:216283 ; RRID:Addgene_216283)

  • For your References section:

    SpyMask enables combinatorial assembly of bispecific binders. Driscoll CL, Keeble AH, Howarth MR. Nat Commun. 2024 Mar 16;15(1):2403. doi: 10.1038/s41467-024-46599-9. 10.1038/s41467-024-46599-9 PubMed 38493197
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