pEGFP-C2-DR
(Plasmid
#216266)
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PurposeDox-responsive module fused with EGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216266 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C2
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Backbone manufacturerClontech/Takara
- Total vector size (bp) 6064
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCodon-optimized TetR - Lipid binding motif (KRf - KLK) - Codon-optimized TetR
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Insert Size (bp)1284
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C2-DR was a gift from Susumu Tomita (Addgene plasmid # 216266 ; http://n2t.net/addgene:216266 ; RRID:Addgene_216266) -
For your References section:
Chemogenetic regulation of the TARP-lipid interaction mimics LTP and reversibly modifies behavior. Park J, Berthoux C, Hoyos-Ramirez E, Shan L, Morimoto-Tomita M, Wang Y, Castillo PE, Tomita S. Cell Rep. 2023 Aug 29;42(8):112826. doi: 10.1016/j.celrep.2023.112826. Epub 2023 Jul 19. 10.1016/j.celrep.2023.112826 PubMed 37471228