pLV_CMV_Prss-Halo-KDEL
(Plasmid
#216256)
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PurposeCMV promoter-regulated ER targeted HaloTag expression vector
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216256 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV
- Backbone size w/o insert (bp) 7922
- Total vector size (bp) 8926
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameER retained HaloTag
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SpeciesBacterial
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Insert Size (bp)1003
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV_CMV_Prss-Halo-KDEL was a gift from Edward Avezov (Addgene plasmid # 216256 ; http://n2t.net/addgene:216256 ; RRID:Addgene_216256) -
For your References section:
Luminal transport through intact endoplasmic reticulum limits the magnitude of localized Ca(2+) signals. Crapart CC, Scott ZC, Konno T, Sharma A, Parutto P, Bailey DMD, Westrate LM, Avezov E, Koslover EF. Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2312172121. doi: 10.1073/pnas.2312172121. Epub 2024 Mar 19. 10.1073/pnas.2312172121 PubMed 38502705