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pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL
(Plasmid #216253)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216253 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone size w/o insert (bp) 7715
  • Total vector size (bp) 9782
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ER-targeted D4 second generation cameleon (FRET calcium sensor)
  • Alt name
    D4ER
  • Species
    Synthetic
  • Insert Size (bp)
    2067
  • Promoter TRE3G
  • Tags / Fusion Proteins
    • mTurquoise (N terminal on insert)
    • REAch (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL was a gift from Edward Avezov (Addgene plasmid # 216253 ; http://n2t.net/addgene:216253 ; RRID:Addgene_216253)

  • For your References section:

    Luminal transport through intact endoplasmic reticulum limits the magnitude of localized Ca(2+) signals. Crapart CC, Scott ZC, Konno T, Sharma A, Parutto P, Bailey DMD, Westrate LM, Avezov E, Koslover EF. Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2312172121. doi: 10.1073/pnas.2312172121. Epub 2024 Mar 19. 10.1073/pnas.2312172121 PubMed 38502705
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