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pLV_TRE3G_hRTN3E-Halo
(Plasmid #216252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone manufacturer
    VectorBuilder
  • Total vector size (bp) 11700
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    reticulon-3 isoform e
  • Alt name
    RTN3E
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4008
  • GenBank ID
    NM_001265589
  • Entrez Gene
    RTN3 (a.k.a. ASYIP, HAP, NSPL2, NSPLII, RTN3-A1)
  • Promoter TRE3G
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV_TRE3G_hRTN3E-Halo was a gift from Edward Avezov (Addgene plasmid # 216252 ; http://n2t.net/addgene:216252 ; RRID:Addgene_216252)

  • For your References section:

    Luminal transport through intact endoplasmic reticulum limits the magnitude of localized Ca(2+) signals. Crapart CC, Scott ZC, Konno T, Sharma A, Parutto P, Bailey DMD, Westrate LM, Avezov E, Koslover EF. Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2312172121. doi: 10.1073/pnas.2312172121. Epub 2024 Mar 19. 10.1073/pnas.2312172121 PubMed 38502705
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