Skip to main content
Addgene

pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag
(Plasmid #216250)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216250 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2800
  • Total vector size (bp) 6158
  • Vector type
    Mammalian Expression, CRISPR ; HDR template
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDR template for human SEC61B
  • Alt name
    SEC61B
  • Species
    H. sapiens (human), Synthetic; HDR template
  • Insert Size (bp)
    2800
  • GenBank ID
    NM_006808.3
  • Entrez Gene
    SEC61B
  • Tag / Fusion Protein
    • BSD.P2A.miniIAA7.3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer GTGGACTCTTGTTCCAAACTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag was a gift from Elina Ikonen (Addgene plasmid # 216250 ; http://n2t.net/addgene:216250 ; RRID:Addgene_216250)

  • For your References section:

    HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells. Li S, Wang Y, van der Stoel M, Zhou X, Madhusudan S, Kanerva K, Nguyen VD, Eskici N, Olkkonen VM, Zhou Y, Raivio T, Ikonen E. Genome Biol. 2024 Feb 26;25(1):58. doi: 10.1186/s13059-024-03187-w. 10.1186/s13059-024-03187-w PubMed 38409044
Related items:
Commonly requested with: