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pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD
(Plasmid #216248)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216248 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2000
  • Total vector size (bp) 4146
  • Vector type
    Mammalian Expression, CRISPR, TALEN ; HDR template
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HDR template for human RANGAP1
  • Alt name
    RANGAP1
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001278651.2
  • Entrez Gene
    RANGAP1 (a.k.a. Fug1, RANGAP, SD)
  • Tag / Fusion Protein
    • miniIAA7.3xFlag.P2A.BSD (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD was a gift from Elina Ikonen (Addgene plasmid # 216248 ; http://n2t.net/addgene:216248 ; RRID:Addgene_216248)

  • For your References section:

    HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells. Li S, Wang Y, van der Stoel M, Zhou X, Madhusudan S, Kanerva K, Nguyen VD, Eskici N, Olkkonen VM, Zhou Y, Raivio T, Ikonen E. Genome Biol. 2024 Feb 26;25(1):58. doi: 10.1186/s13059-024-03187-w. 10.1186/s13059-024-03187-w PubMed 38409044
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