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Addgene

pSIQmate-EGFP
(Plasmid #216241)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216241 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIQmate
  • Backbone size w/o insert (bp) 9001
  • Total vector size (bp) 9729
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Alt name
    the original enhanced GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GCTCCTCCACACGAATTC
  • 3′ sequencing primer TTCTAGAGGCTCGAGAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIQmate-EGFP was a gift from Hyunju Ro (Addgene plasmid # 216241 ; http://n2t.net/addgene:216241 ; RRID:Addgene_216241)

  • For your References section:

    All-in-one IQ toggle switches with high versatilities for fine-tuning of transgene expression in mammalian cells and tissues. Hong J, Sohn KC, Park HW, Jeon H, Ju E, Lee JG, Lee JS, Rho J, Hur GM, Ro H. Mol Ther Methods Clin Dev. 2024 Feb 2;32(1):101202. doi: 10.1016/j.omtm.2024.101202. eCollection 2024 Mar 14. 10.1016/j.omtm.2024.101202 PubMed 38374964
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