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Addgene

FLAG-TRAF6-wt
(Plasmid #21624)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21624 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    Dr. David Russell, UTSW
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNF receptor-associated factor 6
  • Alt name
    TRAF6
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Traf6 (a.k.a. 2310003F17Rik, C630032O20Rik)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-TRAF6-wt was a gift from John Kyriakis (Addgene plasmid # 21624 ; http://n2t.net/addgene:21624 ; RRID:Addgene_21624)

  • For your References section:

    Germinal center kinase is required for optimal Jun N-terminal kinase activation by Toll-like receptor agonists and is regulated by the ubiquitin proteasome system and agonist-induced, TRAF6-dependent stabilization. Zhong J, Kyriakis JM. Mol Cell Biol. 2004 Oct . 24(20):9165-75. 10.1128/MCB.24.20.9165-9175.2004 PubMed 15456887
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