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pCD510B attp BSD
(Plasmid #216236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216236 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-MCS-EF1α-Puro Cloning and Expression Lentivector
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 6544
  • Total vector size (bp) 6943
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    blasticidin S deaminase
  • Alt name
    BSD
  • Species
    Schizosaccharomyces pombe
  • Insert Size (bp)
    399

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TGACCCTGCTTGCTCAAC
  • 3′ sequencing primer TCCACATAGCGTAAAAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD510B attp BSD was a gift from Hyunju Ro (Addgene plasmid # 216236 ; http://n2t.net/addgene:216236 ; RRID:Addgene_216236)

  • For your References section:

    All-in-one IQ toggle switches with high versatilities for fine-tuning of transgene expression in mammalian cells and tissues. Hong J, Sohn KC, Park HW, Jeon H, Ju E, Lee JG, Lee JS, Rho J, Hur GM, Ro H. Mol Ther Methods Clin Dev. 2024 Feb 2;32(1):101202. doi: 10.1016/j.omtm.2024.101202. eCollection 2024 Mar 14. 10.1016/j.omtm.2024.101202 PubMed 38374964
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