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Addgene

pNb4OMT-A53M
(Plasmid #216232)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216232 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 3987
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nb4OMT-A53M
  • Species
    Narcissus sp. aff. pseudonarcissus
  • Insert Size (bp)
    720
  • Mutation
    A53M
  • GenBank ID
    AIL54541.1
  • Promoter P150

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCTCTCCAAGACCGAGCCATCAAC
  • 3′ sequencing primer GAAAGTATAGGAACTTCCAGCCAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNb4OMT-A53M was a gift from Simon d'Oelsnitz & Andrew Ellington (Addgene plasmid # 216232 ; http://n2t.net/addgene:216232 ; RRID:Addgene_216232)

  • For your References section:

    Biosensor and machine learning-aided engineering of an amaryllidaceae enzyme. d'Oelsnitz S, Diaz DJ, Kim W, Acosta DJ, Dangerfield TL, Schechter MW, Minus MB, Howard JR, Do H, Loy JM, Alper HS, Zhang YJ, Ellington AD. Nat Commun. 2024 Mar 7;15(1):2084. doi: 10.1038/s41467-024-46356-y. 10.1038/s41467-024-46356-y PubMed 38453941
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