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Addgene

pSens-4OmeNB
(Plasmid #216231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p15A
  • Backbone size w/o insert (bp) 3760
  • Total vector size (bp) 6083
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    RamR-4NB2
  • Species
    Salmonella typhimurium
  • Insert Size (bp)
    585
  • Mutation
    K64T, L67M, C135D, S138G
  • GenBank ID
    CP001363
  • Promoter J23103

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGATTGTATGGACTTCATCTTCATTACCTC
  • 3′ sequencing primer CGATTGTATGGACTTCATCTTCATTACCTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    744
  • GenBank ID
    ALP48448.1
  • Promoter Pramr

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TGAATATGGCTCGCATCTTATCGAGC
  • 3′ sequencing primer TGAATATGGCTCGCATCTTATCGAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSens-4OmeNB was a gift from Simon d'Oelsnitz & Andrew Ellington (Addgene plasmid # 216231 ; http://n2t.net/addgene:216231 ; RRID:Addgene_216231)

  • For your References section:

    Biosensor and machine learning-aided engineering of an amaryllidaceae enzyme. d'Oelsnitz S, Diaz DJ, Kim W, Acosta DJ, Dangerfield TL, Schechter MW, Minus MB, Howard JR, Do H, Loy JM, Alper HS, Zhang YJ, Ellington AD. Nat Commun. 2024 Mar 7;15(1):2084. doi: 10.1038/s41467-024-46356-y. 10.1038/s41467-024-46356-y PubMed 38453941
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