pcDNA-Dazl-myc
(Plasmid
#21623)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4911
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDazl
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SpeciesR. norvegicus (rat)
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Insert Size (bp)726
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Entrez GeneDazl
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Tags
/ Fusion Proteins
- Myc (C terminal on insert)
- HIS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Day 25 cDNA TD197b/TD198.
Dazl sequence was determined and designed based on coding sequence available for rat genome in 2003. There may be a few discrepancies with the currently available sequence, most notably a C-terminal truncation and point mutation causing P196S.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-Dazl-myc was a gift from David Garbers (Addgene plasmid # 21623 ; http://n2t.net/addgene:21623 ; RRID:Addgene_21623)
