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Addgene

TDP-43 mRuby K84ONBK
(Plasmid #216226)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216226 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone manufacturer
    Novopro
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Transactive response DNA binding protein of 43 kDa
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1968
  • Mutation
    Lysine 84 mutated to a TAG stop codon
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Promoter CMV Promoter

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TDP-43 mRuby K84ONBK was a gift from Jennifer Lee (Addgene plasmid # 216226 ; http://n2t.net/addgene:216226 ; RRID:Addgene_216226)

  • For your References section:

    Genetically encoded lysine photocage for spatiotemporal control of TDP-43 nuclear import. Shadish JA, Lee JC. Biophys Chem. 2024 Jan 23;307:107191. doi: 10.1016/j.bpc.2024.107191. 10.1016/j.bpc.2024.107191 PubMed 38290242
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