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Addgene

NMD2_lenti
(Plasmid #216221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SFFV-tet3G lentiviral backbone
  • Total vector size (bp) 12391
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP P2A RFP_b-globin intron x2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    9066

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NMD2_lenti was a gift from Rebecca Voorhees (Addgene plasmid # 216221 ; http://n2t.net/addgene:216221 ; RRID:Addgene_216221)

  • For your References section:

    Coupled protein quality control during nonsense-mediated mRNA decay. Inglis AJ, Guna A, Galvez-Merchan A, Pal A, Esantsi TK, Keys HR, Frenkel EM, Oania R, Weissman JS, Voorhees RM. J Cell Sci. 2023 May 15;136(10):jcs261216. doi: 10.1242/jcs.261216. Epub 2023 May 23. 10.1242/jcs.261216 PubMed 37218462
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