NMD2
(Plasmid
#216220)
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PurposeNonsense mediated decay GFP P2A RFP reporter with two b-globin introns
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216220 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5
- Total vector size (bp) 9066
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NMD2 was a gift from Rebecca Voorhees (Addgene plasmid # 216220 ; http://n2t.net/addgene:216220 ; RRID:Addgene_216220) -
For your References section:
Coupled protein quality control during nonsense-mediated mRNA decay. Inglis AJ, Guna A, Galvez-Merchan A, Pal A, Esantsi TK, Keys HR, Frenkel EM, Oania R, Weissman JS, Voorhees RM. J Cell Sci. 2023 May 15;136(10):jcs261216. doi: 10.1242/jcs.261216. Epub 2023 May 23. 10.1242/jcs.261216 PubMed 37218462