pLLU2G-Dazl2
(Plasmid
#21622)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21622 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLLU2G
- Backbone size w/o insert (bp) 8264
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Vector typeLentiviral, RNAi, Cre/Lox
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDazl shRNA-2
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SpeciesR. norvegicus (rat)
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Insert Size (bp)54
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer pBluescriptKS
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Dazl shRNA sequence is - 5'-TTTGTTGATCCAGGAGCTGACCTTCAAGAGAGGTCAGCTCCTGGATCAACTTTT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLLU2G-Dazl2 was a gift from David Garbers (Addgene plasmid # 21622 ; http://n2t.net/addgene:21622 ; RRID:Addgene_21622)