Skip to main content
Addgene

2C-EGFP-t2a-mCD4
(Plasmid #216213)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216213 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-N2
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2061
  • Mutation
    CD4 transmembrane domain only
  • Entrez Gene
    Cd4 (a.k.a. L3T4, Ly-4)
  • Promoter MERVL
  • Tag / Fusion Protein
    • GFP-T2A (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer caaagaccccaacgagaagc
  • 3′ sequencing primer agatacattgatgagtttggacaaacc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2C-EGFP-t2a-mCD4 was a gift from Michelle Percharde (Addgene plasmid # 216213 ; http://n2t.net/addgene:216213 ; RRID:Addgene_216213)

  • For your References section:

    Nucleolar-based Dux repression is essential for embryonic two-cell stage exit. Xie SQ, Leeke BJ, Whilding C, Wagner RT, Garcia-Llagostera F, Low Y, Chammas P, Cheung NT, Dormann D, McManus MT, Percharde M. Genes Dev. 2022 Mar 1;36(5-6):331-347. doi: 10.1101/gad.349172.121. Epub 2022 Mar 10. 10.1101/gad.349172.121 PubMed 35273077
Related items: