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Addgene

SGLT1(GFP)-MAP17(nb)
(Plasmid #216210)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216210 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBM
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SGLT1 GFP fusion and MAP17-nanobody fusion
  • Species
    H. sapiens (human)
  • Entrez Gene
    SLC5A2 (a.k.a. SGLT2)
  • Entrez Gene
    PDZK1IP1 (a.k.a. DD96, MAP17, SPAP)
  • Tag / Fusion Protein
    • GFP

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SGLT1(GFP)-MAP17(nb) was a gift from Lei Chen (Addgene plasmid # 216210 ; http://n2t.net/addgene:216210 ; RRID:Addgene_216210)

  • For your References section:

    Structures of human SGLT in the occluded state reveal conformational changes during sugar transport. Cui W, Niu Y, Sun Z, Liu R, Chen L. Nat Commun. 2023 May 22;14(1):2920. doi: 10.1038/s41467-023-38720-1. 10.1038/s41467-023-38720-1 PubMed 37217492
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