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Addgene

pLLU2G-Dazl1
(Plasmid #21621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21621 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLLU2G
  • Backbone size w/o insert (bp) 8264
  • Vector type
    Lentiviral, RNAi, Cre/Lox
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dazl shRNA-1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    54

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer pBluescriptKS
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dazl shRNA sequence is - 5'-TTTGGGCTATGGATTTGTCTCATTCAAGAGATGAGACAAATCCATAGCCCTTTT

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLLU2G-Dazl1 was a gift from David Garbers (Addgene plasmid # 21621 ; http://n2t.net/addgene:21621 ; RRID:Addgene_21621)
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