pBM-strep-UCP1
(Plasmid
#216207)
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Purposefor expression of human strep-tagged UCP1 protein in BacMam system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBM
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUCP1
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SpeciesH. sapiens (human)
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Entrez GeneUCP1 (a.k.a. SLC25A7, UCP)
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Tag
/ Fusion Protein
- strep (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer ggtcttactgacatc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBM-strep-UCP1 was a gift from Lei Chen (Addgene plasmid # 216207 ; http://n2t.net/addgene:216207 ; RRID:Addgene_216207) -
For your References section:
Structural basis for the binding of DNP and purine nucleotides onto UCP1. Kang Y, Chen L. Nature. 2023 Aug;620(7972):226-231. doi: 10.1038/s41586-023-06332-w. Epub 2023 Jun 19. 10.1038/s41586-023-06332-w PubMed 37336486