pLLU2G
(Plasmid
#21620)
-
Purpose(Empty Backbone)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 21620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLL3.7
-
Backbone manufacturerLuk van Parijs
- Backbone size (bp) 7650
-
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
-
Selectable markersEGFP
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)Stbl3
-
Growth instructionsStbl3 30C
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBluescriptKS
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
hUbC promoter was inserted upstream of EGFP.
There are minor sequence discrepancies with depositor's sequence. Depositor is aware of the mismatches and they are acceptable.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLLU2G was a gift from David Garbers (Addgene plasmid # 21620 ; http://n2t.net/addgene:21620 ; RRID:Addgene_21620)