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pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi
(Plasmid #216166)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216166 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV GG hUbV-GFP (EGFP)
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    This plasmid may be prone to recombination, it may be necessary to screen multiple colonies by digest or sequencing.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA-FOXA1-Rank1, sgRNA-FOXA2-Rank2, sgRNA-FOXA2-Rank1, and sgRNA-FOXA3-Rank1
  • gRNA/shRNA sequence
    GCAGCCCGCTCACTTCCCG , AAAGACGAGCGCTTACCTC, AGTAGCTGCTCCAGTCGGA and AGCGCTCTGGATCTCTCAG
  • Species
    H. sapiens (human)
  • Entrez Gene
    FOXA1 (a.k.a. HNF3A, TCF3A)
  • Entrez Gene
    FOXA2 (a.k.a. HNF-3-beta, HNF3B, TCF3B)
  • Entrez Gene
    FOXA3 (a.k.a. FKHH3, HNF3G, TCF3G)
  • Promoter phU6, ph7SK, phH1, pmU6
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Incorporates the use of Addgene ID 216161 (which was generated from Addgene 84034 and 11680)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For difficulties with plasmid recombination, please reach out directly to the depositing laboratory for assistance.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi was a gift from Makiko Iwafuchi (Addgene plasmid # 216166 ; http://n2t.net/addgene:216166 ; RRID:Addgene_216166)

  • For your References section:

    Pioneer and PRDM transcription factors coordinate bivalent epigenetic states to safeguard cell fate. Matsui S, Granitto M, Buckley M, Ludwig K, Koigi S, Shiley J, Zacharias WJ, Mayhew CN, Lim HW, Iwafuchi M. Mol Cell. 2024 Jan 4:S1097-2765(23)01026-2. doi: 10.1016/j.molcel.2023.12.007. 10.1016/j.molcel.2023.12.007 PubMed 38211589
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