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Addgene

TDP-REGv2 Cryptic PE-Max
(Plasmid #216163)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    MUST use stable bacterial line to avoid recombination of UG-rich region (e.g. Stbl3, NEB Stable)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PEMax for prime editing with internal cryptic exon

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    David Liu (pCMV-PEmax ; Addgene plasmid # 174820 ; http://n2t.net/addgene:174820 ; RRID:Addgene_174820). The TDP-REG cryptic exon was inserted into this vector using Gibson assembly.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.15.565967 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TDP-REGv2 Cryptic PE-Max was a gift from Pietro Fratta (Addgene plasmid # 216163 ; http://n2t.net/addgene:216163 ; RRID:Addgene_216163)

  • For your References section:

    Creation of de novo cryptic splicing for ALS/FTD precision medicine. Wilkins OG, Chien MZYJ, Wlaschin JJ, Pisliakova M, Thompson D, Digby H, Simkin RL, Diaz JA, Mehta PR, Keuss MJ, Zanovello M, Brown AL, Harley P, Darbey A, Karda R, Fisher EMC, Cunningham TJ, Le Pichon CE, Ule J, Fratta P. bioRxiv. 2023 Nov 15:2023.11.15.565967. doi: 10.1101/2023.11.15.565967. Preprint. 10.1101/2023.11.15.565967 PubMed 38014203
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