TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV
(Plasmid
#216162)
-
PurposeExpresses Cre recombinase in cells lacking TDP-43 function
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 216162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepTwist CMV
-
Vector typeMammalian Expression, Cre/Lox
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Growth instructionsMUST use stable bacterial line to avoid recombination of UG-rich region (e.g. Stbl3, NEB Stable)
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.15.565967 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV was a gift from Pietro Fratta (Addgene plasmid # 216162 ; http://n2t.net/addgene:216162 ; RRID:Addgene_216162) -
For your References section:
Creation of de novo cryptic splicing for ALS/FTD precision medicine. Wilkins OG, Chien MZYJ, Wlaschin JJ, Pisliakova M, Thompson D, Digby H, Simkin RL, Diaz JA, Mehta PR, Keuss MJ, Zanovello M, Brown AL, Harley P, Darbey A, Karda R, Fisher EMC, Cunningham TJ, Le Pichon CE, Ule J, Fratta P. bioRxiv. 2023 Nov 15:2023.11.15.565967. doi: 10.1101/2023.11.15.565967. Preprint. 10.1101/2023.11.15.565967 PubMed 38014203