pLLU2G-Oct3
(Plasmid
#21616)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLLU 2G
- Backbone size w/o insert (bp) 7861
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Vector typeLentiviral, RNAi
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOct3 shRNA
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Alt nameOct4, Pou5f1
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Insert Size (bp)54
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site HpaI (destroyed during cloning)
- 5′ sequencing primer hUBCpro-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Oct3 shRNA sequence is - 5'-TTGTTTGAACCGTGTGAGGTGGAACTTCAAGAGAGTTCCACCTCACACGGTTCT
This is a 3rd generation lentiviral plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLLU2G-Oct3 was a gift from David Garbers (Addgene plasmid # 21616 ; http://n2t.net/addgene:21616 ; RRID:Addgene_21616) -
For your References section:
Spermatogonial stem cell self-renewal requires OCT4, a factor downregulated during retinoic acid-induced differentiation. Dann CT, Alvarado AL, Molyneux LA, Denard BS, Garbers DL, Porteus MH. Stem Cells. 2008 Nov . 26(11):2928-37. 10.1634/stemcells.2008-0134 PubMed 18719224
