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Addgene

FLAG-TRX
(Plasmid #21615)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21615 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    Dr. David Russell, UTSW
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    thioredoxin
  • Alt name
    TXN
  • Species
    H. sapiens (human)
  • Entrez Gene
    TXN (a.k.a. TRDX, TRX, TRX1, TXN1, Trx80)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-TRX was a gift from John Kyriakis (Addgene plasmid # 21615 ; http://n2t.net/addgene:21615 ; RRID:Addgene_21615)

  • For your References section:

    Activation of apoptosis signal-regulating kinase 1 (ASK1) by tumor necrosis factor receptor-associated factor 2 requires prior dissociation of the ASK1 inhibitor thioredoxin. Liu H, Nishitoh H, Ichijo H, Kyriakis JM. Mol Cell Biol. 2000 Mar . 20(6):2198-208. 10.1128/MCB.20.6.2198-2208.2000 PubMed 10688666
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