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Addgene

pBi2FC-C-GRF4-P2A:mTagBFP2
(Plasmid #216140)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216140 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGWB502-omega
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GRF4 isoform 1
  • Species
    A. thaliana (mustard weed)
  • GenBank ID
    AT1G35160.1

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GRF4 - Transcript isoform AT1G35160.1 is used in this plasmid.

Please visit https://www.biorxiv.org/content/10.1101/2024.06.28.601222v1.full for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBi2FC-C-GRF4-P2A:mTagBFP2 was a gift from Imran Siddiqi (Addgene plasmid # 216140 ; http://n2t.net/addgene:216140 ; RRID:Addgene_216140)
  • For your References section:

    Improved validation of protein interactions using bicistronic BiFC (Bi2FC). Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I. Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 10.1007/s12298-024-01477-y. Epub 2024 Jul 2. 10.1007/s12298-024-01477-y PubMed 39100877