pBi2FC-N-MPK6-P2A:mCherry
(Plasmid
#216137)
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PurposeControls for Bicistronic BiFC
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216137 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGWB502-omega
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMPK6
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SpeciesA. thaliana (mustard weed)
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Entrez GeneMPK6 (a.k.a. AT2G43790, ATMAPK6, ATMPK6, F18O19.10, MAP KINASE 6, MAP kinase 6, MAPK6)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2024.06.28.601222v1.full for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBi2FC-N-MPK6-P2A:mCherry was a gift from Imran Siddiqi (Addgene plasmid # 216137 ; http://n2t.net/addgene:216137 ; RRID:Addgene_216137) -
For your References section:
Improved validation of protein interactions using bicistronic BiFC (Bi2FC). Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I. Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 10.1007/s12298-024-01477-y. Epub 2024 Jul 2. 10.1007/s12298-024-01477-y PubMed 39100877