lenti-mito-mTagBFP2
(Plasmid
#216130)
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PurposeLentiviral vector for expressing mTagBFP2 localizing to mitochondria.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 216130 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonelentiCas9-Blast (Plasmid #52962)
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Backbone manufacturerFeng Zhang lab
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEF1a-COX8AmitochondrialLocalizationSignal-mTagBFP2-WPRE
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lenti-mito-mTagBFP2 was a gift from Stefan Kubicek (Addgene plasmid # 216130 ; http://n2t.net/addgene:216130 ; RRID:Addgene_216130) -
For your References section:
Pooled multicolour tagging for visualizing subcellular protein dynamics. Reicher A, Reinis J, Ciobanu M, Ruzicka P, Malik M, Siklos M, Kartysh V, Tomek T, Koren A, Rendeiro AF, Kubicek S. Nat Cell Biol. 2024 May;26(5):745-756. doi: 10.1038/s41556-024-01407-w. Epub 2024 Apr 19. 10.1038/s41556-024-01407-w PubMed 38641660