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Addgene

lenti-ER-mTagBFP2
(Plasmid #216127)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216127 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCas9-Blast (Plasmid #52962)
  • Backbone manufacturer
    Feng Zhang lab
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1a-ProlactinEndoplasmicReticulumSignalSequence-mTagBFP2-KDEL-WPRE
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti-ER-mTagBFP2 was a gift from Stefan Kubicek (Addgene plasmid # 216127 ; http://n2t.net/addgene:216127 ; RRID:Addgene_216127)

  • For your References section:

    Pooled multicolour tagging for visualizing subcellular protein dynamics. Reicher A, Reinis J, Ciobanu M, Ruzicka P, Malik M, Siklos M, Kartysh V, Tomek T, Koren A, Rendeiro AF, Kubicek S. Nat Cell Biol. 2024 May;26(5):745-756. doi: 10.1038/s41556-024-01407-w. Epub 2024 Apr 19. 10.1038/s41556-024-01407-w PubMed 38641660
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