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YIplac128-TEF2p-LDH-CYC1t
(Plasmid #216120)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216120 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YIplac128-TEF2p-CYC1t
  • Backbone manufacturer
    Valeria Mapelli
  • Backbone size w/o insert (bp) 4981
  • Total vector size (bp) 5980
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lactate dehydrogenase
  • Alt name
    LDH
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    999
  • Entrez Gene
    LDH
  • Promoter TEF2 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer TCTAATATATAAATTCTCTTGCATTTT
  • 3′ sequencing primer GGGACCTAGACTTCAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YIplac128-TEF2p-LDH-CYC1t was a gift from Yvonne Nygård (Addgene plasmid # 216120 ; http://n2t.net/addgene:216120 ; RRID:Addgene_216120)

  • For your References section:

    Engineering of Saccharomyces cerevisiae for enhanced metabolic robustness and L-lactic acid production from lignocellulosic biomass. Choi B, Tafur Rangel A, Kerkhoven EJ, Nygard Y. Metab Eng. 2024 Jul;84:23-33. doi: 10.1016/j.ymben.2024.05.003. Epub 2024 May 23. 10.1016/j.ymben.2024.05.003 PubMed 38788894
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