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Addgene

pIE2-DBD
(Plasmid #21602)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21602 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIZT
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4628
  • Vector type
    Insect Expression ; Gateway Destination Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Tetracycline, 25 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAL4 DBD
  • Alt name
    GAL4 DNA binding domain
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    591
  • GenBank ID
    NP_015076
  • Entrez Gene
    GAL4 (a.k.a. YPL248C, GAL81)
  • Tags / Fusion Proteins
    • 3xHA (N terminal on backbone)
    • Gateway destination cassette (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TTATCGCGCCTATAAATACAGCCCGCAAC
  • 3′ sequencing primer CACGCGCTTGAAAGGAGTGTGTAAATGGAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIE2-DBD was a gift from Takahiro Kusakabe (Addgene plasmid # 21602 ; http://n2t.net/addgene:21602 ; RRID:Addgene_21602)

  • For your References section:

    Analysis of protein interactions with two-hybrid system in cultured insect cells. Mon H, Sugahara R, Hong SM, Lee JM, Kamachi Y, Kawaguchi Y, Kusakabe T. Anal Biochem. 2009 May 26. ():. 10.1016/j.ab.2009.05.033 PubMed 19481053
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