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pcDNA3-HA2-Keap1 S3H4
(Plasmid #21594)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21594 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3-HA2
  • Backbone size w/o insert (bp) 5512
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    kelch-like ECH-associated protein 1
  • Alt name
    KEAP1
  • Alt name
    INrf2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1342
  • Mutation
    Mutated residues in the beta-sheet S3 and alpha-helix H4 (KEAP1S3H4 mutant)
  • GenBank ID
    Q14145
  • Entrez Gene
    KEAP1 (a.k.a. INrf2, KLHL19)
  • Tag / Fusion Protein
    • HA2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

S3H4 mutations are - V123A, I125A, G127A, VM160,1AA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA2-Keap1 S3H4 was a gift from Yue Xiong (Addgene plasmid # 21594 ; http://n2t.net/addgene:21594 ; RRID:Addgene_21594)

  • For your References section:

    BTB protein Keap1 targets antioxidant transcription factor Nrf2 for ubiquitination by the Cullin 3-Roc1 ligase. Furukawa M, Xiong Y. Mol Cell Biol. 2005 Jan . 25(1):162-71. 10.1128/MCB.25.1.162-171.2005 PubMed 15601839
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